In man, physiological amounts of vitamin b12 cyanocobalamin are absorbed by the intrinsic factor mediated mechanism exclusively in the. The production of coenzyme b12 using wellcharacterized microorganisms, such as escherichia coli, has recently attracted considerable attention to meet growing demands of coenzyme b12 in various. However, eukaryotes can have both direct and indirect requirements for cobalamin and other corrinoids, and can potentially be further influenced by corrinoiddependent metabolism by their gut. Vitamin b12 is an essential vitamin that is widely used in medical and food industries. Pdf microbial production of vitamin b12 by methanol utilizing. Vitamin b12 synthesis and industrial production cyanocobalamin is one such vitamer in this b complex, since it can easily be metabolized in the body to an active coenzyme form. Pdf microbial production of vitamin b12 by methanol. Bergeys manual ofdeterminative bacteriology 7 and checked against the. Sundaralingam vitamins microorganisms can be used for the commercial production of certain vitamins, such as thiamine, riboflavin, folic. It is estimated that the worlds annual production of vitamin b 12 is around 15,000 kg. In a screening for vitamin b12producing bacteria isolated from indonesian tempeh. A primary uptake phase which was rapid 1 min or less, ph dependent, nontemperature dependent, did not require viable organisms and was.
Species of microbial producers and microbiological processes recommended for the production of vitamin b12. It turns out that vitamin b12, a substance produced by only a few organisms but needed by nearly all of them, wields great power in. For several of these compounds microbiological and algal processes exist, or are rapidly emerging. Microbial synthesis is the only source of commercial production of vitamin b12. Microbial production of vitamin b12 by methanol utilizing strain of pseudomonas specie muhammad riaz1, zumir ahmed ansari1, fouzia iqbal2 and muhammad akram3 1 department of chemistry, university of engineering and technology, lahore 2 pcsir laboratories complex. Production of vitamins by chemical synthesis, or extraction from their known sources has serious disadvantages. Most vitamins are essential for the metabolism of all living organisms, and they are synthe sized by microorganisms and plants. Microbial production of vitamin b12 and antimicrobial activity of. The batch fermentation process was used for the production of vitamin b12 at.
Microbial production of vitamin b12 biotech articles. Cobalamin is an unusual vitamin in that it is not made by plants and instead is synthesized exclusively by bacteria and archaea roth et al. Microbial production of vitamin b12 by methanol utilizing strain of. Vitamins are defined as essential micronu trients that are not synthesized by mammals.
Vitamin b12 biosynthesis is confined to few bacteria and archaea, and as such its production relies on microbial fermentation. In 1948, rickes and colleagues, first time recovered b12 in active crystalline form from actinomycete streptomyces griseus. Its no different for microbes, where scientists have found more evidence that vitamin b12, a substance produced by only a few organisms but needed by nearly all of them, wields great power in the. Bacterial uptake of vitamin b 12 was found to be at least a two stage process. One of the most alluring and fascinating molecules in the world of science and medicine is vitamin b12 cobalamin, which was originally discovered as the. In the microbial world, vitamin b12 is a hot commodity.
Vitamin b12 production by citrobacterfreundii or klebsiella. Vitamin b12 is produced during normal microbial metabolism. Vitamin b12 synthesis by human small intestinal bacteria. Researchers from the uk have identified a new group of microorganisms that may be responsible for much of the worlds vitamin b12 production in the oceans.
High concentrations of vitamin b 12 are detected in sewagesludge solids. The mechanism of bacterial uptake of vitamin b 12, the spectrum of microorganisms capable of such uptake, and the factors involved were the subject of this study. There is no other source or synthetic method known that produce b12 vitamin. Pdf microbial production of vitamin b12 sameera gamage. Microbial production of vitamin b12 by methanol utilizing strain of pseudomonas specie muhammad riaz1, zumir.
308 1401 1497 567 1055 1383 1002 92 1568 128 423 730 606 208 483 504 638 1178 565 1061 189 1279 448 602 1514 848 394 1583 47 638 535 53 269 850 488 710 783 59 1146 1295